Ny strong cytotoxic activity to any in the cell lines, having a moderate cytotoxic activity (30 50 viable cell number) being observed only at the highest tested dose (ten /mL), but with variation involving every single cell line and each and every compound. Also, at low concentrations (0.01 and 0.10 /mL) all four compounds appeared to be stimulatory (120 -140 viable cell number) and so could promote cell proliferation within the HepG2 cell line and, to a lesser extent, in the KATO-III cell line, but not within the otherRelative viable cell quantity ( )Paula M. Kustiawan et al./Asian Pac J Trop Biomed 2014; four(7): 549-A160 140 120 100 80 60 40 20Relative viable cell quantity ( )100 80 60 40 20 0 0.001 0.Relative viable cell quantity ( )B140 120 100 80 60 40 20C0.0.Kaempferol 5-Fluorouracil Doxorubicin Relative viable cell number ( )140 120 100 80 60 40 20 0 0.001 0.Naringenin ApigeninConcentration ( /mL)0.CAPENaringeninConcentration ( /mL) Apigenin E0.0.0.Relative viable cell number ( )DKaempferol120 one hundred 80 60 40 205-Fluorouracil DoxorubicinCAPENaringeninConcentration ( /mL) Apigenin0.Kaempferol 5-Fluorouracil DoxorubicinCAPEKaempferol 5-Fluorouracil DoxorubicinNaringenin ApigeninConcentration ( /mL)0.Methyl 4-chloro-3-oxobutanoate Chemscene 0.148256-82-0 web 0.Concentration ( /mL) Naringenin Apigenin0.CAPEKaempferol 5-Fluorouracil DoxorubicinCAPEFigure 1. The sensitivities of diverse cell lines to every in the six tested pure compounds. Relative viable cell number of the (A) HepG2, (B) SW620, (C) ChaGo-I, (D) KATO-III and (E) BT474 cell lines immediately after a 48 h therapy using the indicated pure compounds and concentrations. The information are shown because the meanSD percentage of viable cell numbers relative to that of your control, as determined by the surrogate MTT assay, and are derived from three replications.three cell lines. Excluding the highest tested dose (ten / mL), no important cytotoxicity was observed with any of those four compounds against any of your cell lines except for BT-474, which, in contrast, showed a moderate to weak degree of cytotoxic activity (50 -70 viable cell number) against four compounds at all tested doses (0.01-10.00 /mL). 4. Discussion Apis sp., the honey bees) solutions exert a fairly diverse array of bioactivities, which includes anti-proliferation of cancer cell lines. Having said that, so far really handful of research have addressed the merchandise of stingless bees, and none on the solution of stingless bees native to Indonesia. In Thailand, antimicrobial and antiproliferative activities have been reported in the crude extract and partial purified fractions of Tetragonula laeviceps propolis but the chemical structure from the active compounds has not been reported [22,23] .PMID:28038441 Nonetheless, the key compounds in the geopropolis (propolis mixing with wax and soil in its constitution) of your stingless bee, Melipona scutellaris, in B razil have been identified as benzophenones, whilst, in contrast to honey bee propolis, flavonoids had been absent. The geopropolis also had each antimicrobial and antiproliferative activities[24]. In this study, 5 various human cancer derived cell lines had been discovered to be differentially sensitive when it comes to the cytotoxic activity to the crude extracts of unique bee products and from diverse species. General, the HepG(mostlyThere are numerous published research suggesting that beecell line was sensitive to the CMEH and CEEH extracts from all four tested stingless bee species. The crude extracts of propolis from T. incisa and T. fuscobalteata had been cytotoxic against many of the five cell lines, whilst the crude bee.