Adding one hundred lL of 0.2 N Folin iocalteu’s phenol reagent andTable 1 Cytotoxicity in the medicinal plant extracts against H1N1 virus.S. No. Name Toxicity on the extracts IC50 1 2 3 4 five 6 7 eight 9 10 11 MP-L1 MP-s1 MP-L2 MP-s2 MP-L3 MP-s3 MP-L4 MP-s4 MP-L5 MP-s5 Oseltamivir 46.69 22.43 60.09 33.98 33.36 23.60 ND ND 65.99 20.50 six.44 CC50 1026.07 100 100 50 20 40 50 three.95 100 18.30 one hundred TI 21.97 four.45 1.66 1.47 0.59 0.59 ND ND 0.60 0.89 15.Handle drug Oseltamivir; IC50 inhibitory concentration of 50 ; CC50 cytotoxicity concentration of 50 ; TI therapeutic index.Leaf and stem bark extracts against H1NFigure 3 Comparative analysis in between leaves and stem bark extracts of selected medicinal plants for their anti-viral activity against H1N1 by SRB assay. MP medicinal plants; control drug Oseltamivir.80 lL of saturated sodium carbonate within a 96 well plate containing 20 lL of methanol extract. The optical density of the samples was measured immediately after 1 h of incubation at 750 nm. The calculations have been produced based on the values obtained for gallic acid at distinctive concentrations and expressed in mg/g of sample. two.9. Free of charge radical scavenging activity The 1,1-diphenyl-2-picrylhydrazyl (DPPH) and two,20 -azinobis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging activity of the samples was measured by following the procedures of Maria John et al. (2014, 2015). 20 lL of methanol extract was mixed with 180 lL of DPPH (0.5 mM) and ABTS reagents had been incubated for 20 min and 7 min. The absorbance was measured at 515 nm and 750 nm for the respective analysis making use of a micro plate reader.2.ten. Secondary metabolite analysis by HPLC The methanol extracts have been analysed by HPLC (Agillent 1100, USA) to seek out the metabolic variations. Water and acetonitrile containing 0.1 formic acid served as mobile phases A and B with the flow rate of 1 ml/min. The samples had been analysed making use of C18 column having a diode array detector (DAD) at 254 nm. Total run time of your sample was 40 min and determined by the individual requirements retention time (tR) the metabolite identification was made. 2.11. Data evaluation Data evaluation was performed using distinct softwares which include Statistica 7 for metabolite comparison, heat map for metabolite correlation studies and SPSS for statistical evaluation of the biochemical analysis. For the individual metabolite evaluation,536 log10 values have been used for their comparison amongst the samples. three. Benefits and discussion three.1. Total phenolic and flavonoid contents of the medicinal plants Total flavonoid and polyphenol contents from the medicinal plant extracts have been compared and are presented in Fig. 1. Results revealed that the flavonoid content was the highest for MP-s2 (24.82 mg/g) followed by MP-L5 (22.30 mg/g). MP-s5 (11.36 mg/g) followed by MP-L3 (11.88 mg/g) had the lowest flavonoid content amongst the samples analysed.279236-77-0 supplier Among the 5 plants tested, the stem bark showed a higher flavonoid content with MP-s2, MP-s3 and MP-s4 when in comparison with their leaves (MP-L2, MP-L3 and MP-L4).Buy1355070-36-8 Inside the case of total polyphenol content, MP-s2 registered a higher (29.PMID:24458656 73 mg/g) phenolic content material followed by MP-L1. Mp-s5 and MP-s4 registered the lowest content material when it comes to polyphenols. Right here the stem bark of MP-s2 and s3 showed a high phenolic content than that of their leaves (Fig. 1B). 3.2. Free of charge radical scavenging potential on the selected medicinal plants The leaves and stem bark of your chosen medicinal plants were compared for their antioxidant prospective applying DPPH and ABTS assay.